Journal: Nature Communications

Article Title: A macrophage-induced subpopulation of mesenchymal cells expressing Fcer1g contributes to wound-induced fibrosis

doi: 10.1038/s41467-026-69449-2

Figure Lengend Snippet: a Differentially expressed (DE) genes in subcluster 9 identified using DESeq2 (Wald test, two-sided; Benjamini-Hochberg adjustment for multiple comparisons; DE: adjusted P -value < 0.05; Non-DE: adjusted P -value ≥ 0.05). b Split UMAP showing Fcer1g expression in mesenchymal subclusters from 3 dpi wounds treated with vehicle or AP20187. c Representative immunofluorescence image showing channel-splitted and merged GFP, FCER1G, and PDGFRα staining in vehicle-treated MaFIA wounds at 3 dpi ( n = 3). White arrow: GFP + cells; pink arrow: FCER1G + PDGFRα + cells. d ,Representative immunofluorescent images of skin sections stained for FCER1G and PDGFRα during unwounded and wounded stages ( n = 2 for unwounded, 15 dpi, and 21 dpi; n = 3 for 3 dpi and 7 dpi). White arrow: FCER1G + PDGFRα + cells. e Stacked bar graph showing the proportional distribution of FCER1G + PDGFRα + cells across PDGFRα + cells and total cells. f , g Comparison of vehicle and AP20187-treated MaFIA wounds at 3 dpi. f Representative immunofluorescence images of FCER1G and PDGFRα staining.White arrow: FCER1G + PDGFRα + cells. g Quantitative analysis of FCER1G + PDGFRα + cells as a percentage of total PDGFRα + cells ( n = 3 per condition, unpaired two-sided t test, P = 0.0114). h Representative histogram showing Fcer1g-PE fluorescence intensity in PDGFRα + cells from 3 dpi MaFIA wounds treated with vehicle and AP20187 ( n = 3 per condition). i – k NIH/3t3 MDF cultured with or without bone-marrow-derived macrophages isolated from MaFIA mice. i Representative microscopic images showing the MDF-macrophage co-culture. Blue: CellTrace TM labeled MDF; Green: GFP + macrophage isolated from MaFIA mice. j Representative FACS plot showing the Live/CTV + FCER1G + PDGFRα + cells under MDF-macrophage co-culture. k Quantification of FCER1G + PDGFRα + cells out of total MDF ( n = 3 per condition, unpaired two-sided t test, P = 0.0008). Data are presented as mean ± s.d.

Article Snippet: Antibodies used in this study include PDGFRA-APC (Invitrogen, Cat# 17-1401-81, 1:50), FCER1G-PE (Miltenyi Biotec, Cat# 130-118-760, 1:200), F4/80-AF647 (BD Biosciences, Cat# 565853, 1:100) and CD11b-PE/Cy7 (BioLegend, Cat# 101216, Clone#M1/70, 1:100).

Techniques: Expressing, Immunofluorescence, Staining, Comparison, Fluorescence, Cell Culture, Derivative Assay, Isolation, Co-Culture Assay, Labeling

Journal: Nature Communications

Article Title: A macrophage-induced subpopulation of mesenchymal cells expressing Fcer1g contributes to wound-induced fibrosis

doi: 10.1038/s41467-026-69449-2

Figure Lengend Snippet: a Schematic of the Pdgfra-rtTA; Fcer1g-Cre; Dragon-DTA mouse, which allows DTA-mediated ablation of Fcer1g -and Pdgfrα -expressing cells upon Dox administration. b , c Depletion efficiency of Fcer1g -expressing cells in Pdgfra-rtTA; Fcer1g-Cre; Dragon-DTA mice. b Representative immunofluorescent images of 7 dpi wounds in control and depleted groups. White arrow: FCER1G + PDGFRα + cells. c Quantitative analysis of FCER1G + PDGFRα + mesenchymal cells per high-power field (HPF) at 7 dpi ( n = 3 per group, unpaired two-sided t test, P = 0.0129). d , Representative macroscopic images of control and depleted wounds over time (control: n = 11, depleted: n = 8 for all time points). e Wound area expressed as a percentage of the wound area immediately post-injury (control: n = 11, depleted: n = 8 for all time points). f , g Macroscopic analysis of scar area in control and depleted groups at ( f ) 21 dpi (control: n = 12, depleted: n = 11, unpaired two-sided t test, P < 0.0001) and ( g ) 8 wpi ( n = 11 per group, unpaired two-sided t test, P = 0.0011). h H&E staining of wounds at different time points (7 dpi: control n = 4, depleted n = 5; 11 dpi: n = 6 per group; 21 dpi: n = 11 per group; 8 wpi: n = 11 per group; 50 μm from the center of the wound). i, Scar index (scar area/average thickness, µm) of 21 dpi wounds in control and depleted groups (5 slides per n, n = 10 per group, unpaired two-sided t test, P = 0.0190). j Total collagen quantified from 21 dpi wounds in control and depleted groups (control: n = 7, depleted: n = 5; unpaired two-sided t test, P = 0.0308). k t-SNE plot visualizing ECM organization in unwounded skin and wounds from control and depleted groups at 8 wpi; clusters for each condition are highlighted with shaded regions (unwounded: n = 19, control: n = 18, depleted: n = 18). Data are presented as mean ± s.d.

Article Snippet: Antibodies used in this study include PDGFRA-APC (Invitrogen, Cat# 17-1401-81, 1:50), FCER1G-PE (Miltenyi Biotec, Cat# 130-118-760, 1:200), F4/80-AF647 (BD Biosciences, Cat# 565853, 1:100) and CD11b-PE/Cy7 (BioLegend, Cat# 101216, Clone#M1/70, 1:100).

Techniques: Expressing, Control, Staining

Journal: Nature Communications

Article Title: A macrophage-induced subpopulation of mesenchymal cells expressing Fcer1g contributes to wound-induced fibrosis

doi: 10.1038/s41467-026-69449-2

Figure Lengend Snippet: a – c EdU incorporation in FCER1G + PDGFRα + and FCER1G - PDGFRα + cells from 3 dpi wounds. a Representative histogram of EdU-488 fluorescence intensity. b Quantitative data of EdU + cells as a percentage of total live cells ( n = 3 per condition, paired two-sided t test, P = 0.0017). c: Quantitative data of median fluorescence intensity of EdU ( n = 3 per condition, paired two-sided t test, P = 0.0005). d , e Boyden chamber assay measuring the migration ability of FCER1G + PDGFRα + and FCER1G - PDGFRα + cells from 3 dpi wounds. d Representative images of migrated cells stained with crystal violet. e Quantitative analysis of the number of migrated cells ( n = 6 per condition, paired two-sided t test, P = 0.0148). Data are presented as mean ± s.d.

Article Snippet: Antibodies used in this study include PDGFRA-APC (Invitrogen, Cat# 17-1401-81, 1:50), FCER1G-PE (Miltenyi Biotec, Cat# 130-118-760, 1:200), F4/80-AF647 (BD Biosciences, Cat# 565853, 1:100) and CD11b-PE/Cy7 (BioLegend, Cat# 101216, Clone#M1/70, 1:100).

Techniques: Fluorescence, Boyden Chamber Assay, Migration, Staining

Journal: Nature Communications

Article Title: A macrophage-induced subpopulation of mesenchymal cells expressing Fcer1g contributes to wound-induced fibrosis

doi: 10.1038/s41467-026-69449-2

Figure Lengend Snippet: a UMAP visualization of PDGFRA⁺PDGFRB⁺ mesenchymal subclusters from the human skin wound healing scRNA-seq dataset ( GSE241132 ). b , c Expression of a mouse-derived Fcer1g mesenchymal gene signature (top 25 genes with highest uniqueness scores) in human mesenchymal cells, calculated using Seurat’s AddModuleScore(). b Feature plot. c Violin plot. d , e Temporal dynamics of human mesenchymal subcluster 11 across days post injury (0, 1, 7, and 30 dpi). d Feature plots showing subcluster 11 distribution. e Quantification of subcluster 11 as a percentage of total mesenchymal cells, ( n = 3 per condition, One-way ANOVA with Dunnett’s post-hoc test for multiple comparison against 7 dpi. D7 vs. D0 Padj = 0.098; D7 vs. D1 Padj = 0.023; D7 vs. D30 Padj = 0.233). f Cross-species correlation of differentially expressed genes (DEGs) in FCER1G-expressing mesenchymal cells between mouse and human, based on Seurat FindMarkers() output. Spearman’s correlation coefficient and p-value are shown. g Normalized ssGSEA enrichment scores of mice Fcer1g signature genes (top 100 by log fold change) across wound states in human bulk RNA-seq dataset GSE178411 , including normal skin ( n = 24), early wound ( n = 22), late wound ( n = 29), chronic wound ( n = 3), and hypertrophic scar ( n = 28) (One-way ANOVA with Dunnett’s post-hoc test for multiple comparisons against normal skin. Padj < 0.0001 for all comparisons). Data are presented as mean ± s.d.

Article Snippet: Antibodies used in this study include PDGFRA-APC (Invitrogen, Cat# 17-1401-81, 1:50), FCER1G-PE (Miltenyi Biotec, Cat# 130-118-760, 1:200), F4/80-AF647 (BD Biosciences, Cat# 565853, 1:100) and CD11b-PE/Cy7 (BioLegend, Cat# 101216, Clone#M1/70, 1:100).

Techniques: Expressing, Derivative Assay, Comparison, RNA Sequencing